Prof. Christoph A. Klein, MD

Division head

Contact info:

Address:

Division of Oncogenomics

University of Regensburg

Franz-Josef-Strauss-Allee 11
93053 Regensburg
Germany

Phone: +49 (0)941 944 6720

Fax: +49 (0)941 944 6719

E-mail: Christoph.Klein@klinik.uni-regensburg.de

2004

1995

2006-

2001-2006

1997-2001

1995

2007

2001

1999

1994-1995

1. Stoecklein NH, Klein CA. Genetic disparity between primary tumours, disseminated tumour cells, and manifest metastasis. Int J Cancer. 2009 Sep 30. [Epub ahead of print]

2. Klein CA, Stoecklein NH. Lessons from an Aggressive Cancer: Evolutionary Dynamics in Esophageal Carcinoma. Cancer Res. 2009 Jul 1;69(13):5285-8.

3. Hafner C, Stoehr R, van Oers JM, Zwarthoff EC, Hofstaedter F, Klein C, Landthaler M, Hartmann A, Vogt T. The Absence of BRAF, FGFR3, and PIK3CA Mutations Differentiates Lentigo Simplex from Melanocytic Nevus and Solar Lentigo. J Invest Dermatol. 2009 Nov;129(11):2730-5.

4. Dango S, Wang XT, Gold M, Cucuruz B, Klein CA, Passlick B, Sienel W. Expression of melanoma-antigen-A (MAGE-A) in disseminated tumor cells in regional lymph nodes of patients with operable non-small cell lung cancer. Lung Cancer. 2009 May 19. [Epub ahead of print]

5. Klein CA. Parallel progression of primary tumours and metastases. Nat Rev Cancer. 2009 Apr;9(4):302-12.

6. Imle A, Polzer B, Alexander S, Klein CA, Friedl P. Genomic instability of micronucleated cells revealed by single-cell comparative genomic hybridization. Cytometry A. 2009 Jul;75(7):562-8.

7. Gužvić M, Klein CA. The biology of cancer metastasis. In The Impact of Tumor Biology on Cancer Treatment and Multidisciplinary Strategies, edited by Molls M, Vaupel P, Nieder C, Anscher MS. 2009; p.117-128. Springer, Berlin, Heidelberg.

8. Weckermann D, Polzer B, Ragg T, Blana A, Schlimok G, Arnholdt H, Bertz S, Harzmann R, Klein CA. Perioperative Activation of Disseminated Tumor Cells in Bone Marrow of Patients With Prostate Cancer. J Clin Oncol. 2009 Apr 1;27(10):1549-56.

9. Gires O, Klein CA, Baeuerle PA. On the abundance of EpCAM on cancer stem cells. Nat Rev Cancer. 22009 Feb;9(2):143.

10. Hüsemann Y, Klein CA. The analysis of metastasis in transgenic mouse. Transgenic Res. 2009 Feb;18(1):1-5.

11. Klein CA. The direct molecular analysis of metastatic precursor cells in breast cancer: A chance for a better understanding of metastasis and for personalised medicine. Eur J Cancer. 2008 Dec;44(18):2721-5.

12. Polzer B, Hartmann CH, Klein CA. Genome and transcriptome analysis of single tumor cells. In Single Cell Analysis: Technologies and Applications, edited by Anselmetti D. 2009; p.235-249. Wiley-VCH Verlag GmbH & Co, KGaA, Weinheim 2009.

13. Klein CA. The Metastasis Cascade. Science. 2008 Sep 26; 321(5897):1785-7.

14. Sienel W, Polzer B, Elshawi K, Lindner M, Morresi-Hauf A, Vay C, Eder F, Passlick B, Klein CA. Cellular localization of EMMPRIN predicts prognosis of patients with operable lung adenocarcinoma independent from MMP-2 and MMP-9. Mod Pathol. 2008 Sep;21(9):1130-8.

15. Stoecklein NH, Hosch SB, Bezler M, Stern F, Hartmann CH, Vay C, Siegmund A, Scheunemann P, Schurr P, Knoefel WT, Verde PE, Reichelt U, Erbersdobler A, Grau R, Ullrich A, Izbicki JR, Klein CA. Direct Genetic Analysis of Single Disseminated Cancer Cells for Prediction of Outcome and Therapy Selection in Esophageal Cancer. Cancer Cell. 2008 May 6;13(5):441-453.

16. Fuhrmann C, Schmidt-Kittler O, Stoecklein NH, Petat-Dutter K, Vay C, Bockler K, Reinhardt R, Ragg T, Klein CA. High-resolution array comparative genomic hybridization of single micrometastatic tumor cells. Nucleic Acids Res. 2008 Apr;36(7):e39.

17. Klein CA, Hartmann CH. Amplification of cDNA from single or rare cells by global PCR (exponential amplification). In Microarrays in Inflammation, edited by Bosio A, Gerstmayer G. 2008; p.81-94. Birkhäuser Verlag, Basel, CH.

18. Hahtola S, Burghart E, Jeskanen L, Karenko L, Abdel-Rahman WM, Polzer B, Kajanti M, Peltomäki P, Pettersson T, Klein CA, Ranki A. Clinicopathological Characterization and Genomic Aberrations in Subcutaneous Panniculitis-Like T-Cell Lymphoma. J Invest Dermatol. 2008 Mar 13; 128(9):2304-09.

19. Hüsemann Y, Geigl JB, Schubert F, Musiani P, Meyer M, Burghart E, Forni G, Eils R, Fehm T, Riethmüller G, Klein CA. Systemic spread is an early step in breast cancer. Cancer Cell. 2008 Jan;13(1):58-68.

20. Hahtola S, Burghart E, Puputti M, Karenko L, Abdel-Rahman WM, Väkevä L, Jeskanen L, Virolainen S, Karvonen J, Salmenkivi K, Kinnula V, Joensuu H, Peltomäki P, Klein CA, Ranki A. Cutaneous T-cell lymphoma-associated lung cancers show chromosomal aberrations differing from primary lung cancer. Genes Chromosomes Cancer. 2007 Nov 5;47(2):107-17.

21. Weckermann D, Polzer B, Klein C. Bedeutung Cytokeratin-positiver Zellen im Knochenmark bei Patienten mit klinisch organbegrenztem Prostatakarzinom. Urologe A. 2007 Sep;46(9):1078-80.

22. Klein CA. Random mutations, selected mutations: A PIN opens the door to new genetic landscapes. Proc Natl Acad Sci U S A. 2006. Nov 28;103(48):18033-4.

23. Hartmann CH, Klein CA. Gene expression profiling of single cells on large-scale oligonucleotide arrays. Nucleic Acids Res. 2006;34(21):e143.

24. Klein CA, Holzel D. Systemic Cancer Progression and Tumor Dormancy: Mathematical Models Meet Single Cell Genomics. Cell Cycle. 2006 Aug 15;5(16):1788-98.

25. Bahar R, Hartmann CH, Rodriguez KA, Denny AD, Busuttil RA, Dolle ME, Calder RB, Chisholm GB, Pollock BH, Klein CA, Vijg J. Increased cell-to-cell variation in gene expression in ageing mouse heart. Nature. 2006 Jun 22;441(7096):1011-4.

26. Schardt JA, Meyer M, Hartmann CH, Schubert F, Schmidt-Kittler O, Fuhrmann C, Polzer B, Petronio M, Eils R, Klein CA. Genomic analysis of single cytokeratin-positive cells from bone marrow reveals early mutational events in breast cancer. Cancer Cell. 2005 Sep;8(3):227-39.

27. Ulmer A, Fischer JR, Schanz S, Sotlar K, Breuninger H, Dietz K, Fierlbeck G, Klein CA. Detection of melanoma cells displaying multiple genomic changes in histopathologically negative sentinel lymph nodes. Clin Cancer Res. 2005 Aug 1;11(15):5425-32.

28. Wang XT, Sienel W, Eggeling S, Ludwig C, Stoelben E, Mueller J, Klein CA, Passlick B. Detection of disseminated tumor cells in mediastinoscopic lymph node biopsies and lymphadenectomy specimens of patients with NSCLC by quantitative RT-PCR. Eur J Cardiothorac Surg. 2005 Jul;28(1):26-32.

29. Klein CA. Single cell amplification methods for the study of cancer and cellular ageing. Mech Ageing Dev. 2005 Jan;126(1):147-51.

30. Stoecklein NH, Luebke AM, Erbersdobler A, Knoefel WT, Schraut W, Verde PE, Stern F, Scheunemann P, Peiper M, Eisenberger CF, Izbicki JR, Klein CA, Hosch SB. Copy number of chromosome 17 but not HER2 amplification predicts clinical outcome of patients with pancreatic ductal adenocarcinoma. J Clin Oncol. 2004 Dec 1;22(23):4737-45. (Erratum in: J Clin Oncol. 2005 Jan 1;23(1):248.)

31. Ulmer A, Schmidt-Kittler O, Fischer J, Ellwanger U, Rassner G, Riethmuller G, Fierlbeck G, Klein CA. Immunomagnetic enrichment, genomic characterization, and prognostic impact of circulating melanoma cells. Clin Cancer Res. 2004 Jan 15;10(2):531-7.

32. Klein CA. Gene expression sigantures, cancer cell evolution and metastatic progression. Cell Cycle. 2004 Jan;3(1):29-31.

33. Klein CA. The systemic progression of human cancer: a focus on the individual disseminated cancer cell - the unit of selection. Adv Cancer Res. 2003;89:35-67.

34. Jentsch I, Geigl J, Klein CA, Speicher MR. Seven-fluorochrome mouse M-FISH for high-resolution analysis of interchromosomal rearrangements. Cytogenet Genome Res. 2003;103(1-2):84-8.

35. Schmidt-Kittler O, Ragg T, Daskalakis A, Granzow M, Ahr A, Blankenstein TJ, Kaufmann M, Diebold J, Arnholdt H, Muller P, Bischoff J, Harich D, Schlimok G, Riethmuller G, Eils R, Klein CA. From latent disseminated cells to overt metastasis: genetic analysis of systemic breast cancer progression. Proc Natl Acad Sci U S A. 2003 Jun 24;100(13):7737-42.

36. Sienel W, Hellers J, Morresi-Hauf A, Lichtinghagen R, Mutschler W, Jochum M, Klein C, Passlick B, Pantel K. Prognostic impact of matrix metalloproteinase-9 in operable non-small cell lung cancer. Int J Cancer. 2003 Feb 20;103(5):647-51.

37. Klein CA. From single disseminated tumor cells to metastasis insights from molecular genetic analyses of single cells. Verh Dtsch Ges Pathol. 2003;87:158-64.

38. Klein CA, Zohlnhöfer D, Petat-Dutter K, Wendler N. Gene expression analysis of a single or few cells. In Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K (editors) Current Protocols in Molecular Biology. 2003; 25B.8.1-25B.8.18. John Wiley & sons, Hoboken, NJ, USA.

39. Klein CA, Blankenstein TJ, Schmidt-Kittler O, Petronio M, Polzer B, Stoecklein NH, Riethmuller G. Genetic heterogeneity of single disseminated tumour cells in minimal residual cancer. Lancet. 2002 Aug 31;360(9334):683-9.

40. Klein CA, Wilke M, Pool J, Vermeulen C, Blokland E, Burghart E, Krostina S, Wendler N, Passlick B, Riethmueller G, Goulmy E. The hematopoietic system-specific minor histocompatibility antigen HA-1 shows aberrant expression in epithelial cancer cells. J Exp Med. 2002 Aug 5;196(3):359-68.

41. Stoecklein NH, Erbersdobler A, Schmidt-Kittler O, Diebold J, Schardt JA, Izbicki JR, Klein CA. SCOMP is superior to degenerated oligonucleotide primed-polymerase chain reaction for global amplification of minute amounts of DNA from microdissected archival tissue samples. Am J Pathol. 2002 Jul;161(1):43-51. (Erratum in: Am J Pathol. 2003 Dec;163(6):2645.)

42. Klein CA, Seidl S, Petat-Dutter K, Offner S, Geigl JB, Schmidt-Kittler O, Wendler N, Passlick B, Huber RM, Schlimok G, Baeuerle PA, Riethmuller G. Combined transcriptome and genome analysis of single micrometastatic cells. Nat Biotechnol. 2002 Apr;20(4):387-92.

43. Klein CA. Direct molecular analysis of single disseminated cancer cells: a prerequisite for the development of adjuvant therapies? Acta Med Austriaca Suppl. 2002;59:10-3.

44. Riethmuller G, Klein CA. Early cancer cell dissemination and late metastatic relapse: clinical reflections and biological approaches to the dormancy problem in patients. Semin Cancer Biol. 2001 Aug;11(4):307-11.

45. Zohlnhofer D, Richter T, Neumann F, Nuhrenberg T, Wessely R, Brandl R, Murr A, Klein CA, Baeuerle PA. Transcriptome analysis reveals a role of interferon-gamma in human neointima formation. Mol Cell. 2001 May;7(5):1059-69.

46. Zohlnhofer D, Klein CA, Richter T, Brandl R, Murr A, Nuhrenberg T, Schomig A, Baeuerle PA, Neumann FJ. Gene expression profiling of human stent-induced neointima by cDNA array analysis of microscopic specimens retrieved by helix cutter atherectomy: Detection of FK506-binding protein 12 upregulation. Circulation. 2001 Mar 13;103(10):1396-402.

47. Klein CA. The biology and analysis of single disseminated tumour cells. Trends Cell Biol. 2000 Nov;10(11):489-93.

48. Riethmuller G, Klein CA, Pantel K. Hunting down the seminal cells of clinical metastases. Immunol Today. 1999 Jul;20(7):294-6.

49. Klein CA, Schmidt-Kittler O, Schardt JA, Pantel K, Speicher MR, Riethmuller G. Comparative genomic hybridization, loss of heterozygosity, and DNA sequence analysis of single cells. Proc Natl Acad Sci U S A. 1999 Apr 13;96(8):4494-9.